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Description:No protozoa were found in any of the animals from P1. They can show that there are differences between various treatments, but they cannot be used to identify key species in a system biology approach [9]. The evolution of the study of rumen microbial diversity is similar to that of other microbial ecosystems, moving from culture-based and microscopic observations to the use of culture-independent, molecular techniques. Furthermore, only OTUs with an average abundance of 0. These parameters allow detection and elimination of smaller, broader peaks that would have a less specific size and not be indicative of single true OTUs. Holotrich protozoa were inoculated by oral administration of 50 mL of holotrich protozoa mix diluted in Coleman's Simplex type Solution [20]. Ammonia NH3 concentration was determined using the phenol method of Whitehead [23].

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